ID   AJ000076; SV 1; linear; genomic DNA; STD; FUN; 2198 BP.
AC   AJ000076;
DT   05-NOV-1997 (Rel. 53, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 5)
DE   Kluyveromyces marxianus epg1 gene encoding endopolygalacturonase, partial
KW   endopolygalacturonase; epg1 gene.
OS   Kluyveromyces marxianus
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
RN   [1]
RC   revised by author 04-NOV-97
RP   1-2198
RA   Siekstele R.;
RT   ;
RL   Submitted (07-JUL-1997) to the EMBL/GenBank/DDBJ databases.
RL   Siekstele R., Gene engineering laboratory, Institute of Biotechnology,
RL   V.Graiciuno 8, 2028, LITHUANIAN REPUBLIC.
RN   [2]
RX   DOI; 10.1002/(SICI)1097-0061(19990315)15:4<311::AID-YEA379>3.0.CO;2-9.
RX   PUBMED; 10206190.
RA   Siekstele R., Bartkeviciute D., Sasnauskas K.;
RT   "Cloning, targeted disruption and heterologous expression of the
RT   Kluyveromyces marxianus endopolygalacturonase gene (EPG1)";
RL   Yeast 15(4):311-322(1999).
FH   Key             Location/Qualifiers
FH
FT   source          1..2198
FT                   /organism="Kluyveromyces marxianus"
FT                   /chromosome="VI"
FT                   /strain="BMK Y-719"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:4911"
FT   TATA_signal     338..352
FT                   /gene="epg1"
FT   CDS             573..>1655
FT                   /codon_start=1
FT                   /gene="epg1"
FT                   /product="endopolygalacturonase"
FT                   /EC_number="3.2.1.15"
FT                   /db_xref="GOA:O13478"
FT                   /db_xref="HSSP:1CZF"
FT                   /db_xref="InterPro:IPR000743"
FT                   /db_xref="InterPro:IPR006626"
FT                   /db_xref="InterPro:IPR011050"
FT                   /db_xref="InterPro:IPR012334"
FT                   /db_xref="UniProtKB/TrEMBL:O13478"
FT                   /protein_id="CAA03900.1"
FT                   /translation="MLFSNTLLIAAASALLAEASPLEKRDSCTLSGKTAGGGLSNCATV
FT                   TVNNVEVPSGKTLDLTGLPDGATVNFVGQVTFGYDEWVGPLVSISGKNIKVVGKSGHLL
FT                   DGDGARWWDGKGDGGKKKPKFMSLRLTGNSDVGGLQIKNTPIQAISVNSCSDTVIHDVT
FT                   IDNRDGDKDNLGHNTDGFDVGNVNNVTIENSHVYNQDDCIAVNSGTGVYFKNNYCSGGH
FT                   GASIGSVGLRSNNVVDTVYFENNQIVNSDNGLRIKTIQKATGSVNNVHFLSNTISGIRK
FT                   FGIVVETDYSNGSTTGTPGSKVPITNFEVDGLTGSVDSSAYRVKIFVAGASKWTWKNVD
FT                   ITGGSSFGSCTGIPSGSGAFC"
FT   sig_peptide     573..657
FT                   /gene="epg1"
FT   mat_peptide     658..>1655
FT                   /gene="epg1"
FT                   /product="endopolygalacturonase"
FT                   /EC_number="3.2.1.15"
SQ   Sequence 2198 BP; 635 A; 441 C; 452 G; 670 T; 0 other;
     gcagccgttt tgcttacagt tctcatgcac aatcagccag attttgcaat agtattaact        60
     tagaattaag gcaacatctt tggatatgca tgtagagtaa gtcgttcgaa accattatta       120
     ttattattat tattattatt attattatta ttattattat tagtattatt gaaattgtta       180
     ttgttcttag tttcactact attattattc atattcatgt tattgacatc gccgaacgac       240
     cagcctccat accgattaga caggatctca aacgtgggct ccagagctca cacattatgc       300
     taaataacta tctactgtaa cagctacaga aaaaaaacta taaaagagcg agggataaac       360
     cactctcttg tgaatcagga tcagtaggta actcataaac cttcttcttt tctctcaaaa       420
     tatcaaataa cagtagtatc aacaacgata tcgaataata ctaactacta caacagtagg       480
     aacagtaacg acaacgacaa cgatagtaac gacaataacg acaccaacaa acaacaggaa       540
     cacagattaa gctcagaaac aaaaaaaaaa aaatgttatt cagcaacacc ttattgatcg       600
     cagcagctag tgcattatta gctgaagctt ctccattgga aaagagagac agttgtacct       660
     tgagtgggaa gacagccgga ggtggtttgt ccaactgtgc cacggtcact gtcaacaacg       720
     tcgaagtccc atctggtaag actttggact tgacaggctt gccagacggt gcgacagtta       780
     atttcgtcgg gcaggttacc tttggttacg atgaatgggt gggtccattg gtctccatct       840
     ccggtaagaa catcaaggtg gtaggtaaat ctggccactt gttagatggt gatggtgcac       900
     gttggtggga cgggaagggt gacggtggta aaaagaagcc taagttcatg agcttgagat       960
     taactggcaa ctcagatgtc ggtgggttgc aaatcaagaa taccccaatt caagctatct      1020
     cagtgaactc ttgtagtgac actgtaattc acgatgtcac cattgacaac agagatggtg      1080
     acaaggacaa cttgggtcac aacactgacg gtttcgatgt tggtaacgtt aacaacgtca      1140
     ccattgagaa cagtcatgtc tacaaccaag atgactgtat cgccgtcaac tccggtaccg      1200
     gtgtctactt caagaacaac tactgttctg gtggtcatgg tgcttccatt ggttcagtcg      1260
     gtcttcgctc aaacaatgtg gttgacaccg tttacttcga gaacaaccaa attgtcaact      1320
     ctgacaacgg tttgagaatt aagaccattc aaaaggccac tggttccgtc aacaacgtgc      1380
     acttcttgtc caacactatc tccggcatca gaaagtttgg tattgttgtt gaaactgatt      1440
     acagcaatgg atccaccacc ggtaccccag gtagcaaggt cccaatcacc aacttcgaag      1500
     tcgatggttt gactggttca gttgactctt ccgcttacag agtcaagatc ttcgttgctg      1560
     gtgcttctaa gtggacttgg aagaatgttg atatcactgg tggttcttct ttcggttcat      1620
     gtactggtat tccatctggt agcggagcct tctgttaagc gtctcttttt attttttttt      1680
     ttttttttat taacgtgaag aagataaggg aagtcttcaa tgcggttctg aatggttgat      1740
     ccatttcgat acctcgggga cttcctttga atatattctg agagtatgac agttggtttt      1800
     ctttctttct ttctattgtt tttgttttta tggaaatata gctttgatga tttaggatat      1860
     tttttgtagt gaaccaatac atgcttgatt aatatacgta cgaggtgggc attctactct      1920
     cattattggt gttttattgg agggaaaaat taaatctagg agtatcgttt agagcgcgaa      1980
     cgtaatatcc atgttcttct ctttgaagag gtcccaccat tgcttcccag atagccagca      2040
     ttcttccatg atattttgcg cttgttttgc actggtgaca ccctttcgaa ccaaagatgt      2100
     caagtgctgc tgatacaaca acctgtattc atacaattct ggatccatca gctcacaatc      2160
     cacagctgaa gatacagaaa atgatacatg tctctgca                              2198
//